Human Hematopoietic Stem Cell Media Search Results


hematopoietic stem cell culture medium  (Cell Applications Inc)
91
Cell Applications Inc hematopoietic stem cell culture medium
a Structures required for AF10 fusion-mediated myeloid transformation. Various AF10 fusion constructs were examined to assess their transforming ability of myeloid progenitors. HA-tag (indicated by red triangles) was fused to MTM and MTMT constructs. FLAG-tag (indicated by blue triangles) was fused to other AF10 fusion constructs. Dotted lines indicate protein-protein interaction. A schema of a myeloid progenitor transformation assay is shown at the top. Hoxa9 expression normalized to Gapdh in first-round colonies (left) is shown as the relative value of CALM-AF10 (arbitrarily set at 100%) (mean of two biological replicates). Colony-forming ability at the third- and fourth-round passages (right) is shown with error bars (mean ± SD of biological replicates, n ≥ 3). b Association of NES-AF10 fusion with ENL in the presence or absence of DOT1L. IP-western blotting (WB) analyses of the chromatin fraction of HEK293T cells [the parental clone or a DOT1L-knockout clone (dDOT1L)] transiently expressing the FLAG-tagged (indicated as f) NES-AF10´ fusion (fNES-AF10´) construct and Xpress-tagged (indicated as x) ENL (xENL) were performed. Co-purification of ENL was observed only in the presence of DOT1L. c Leukemogenesis by NES-ENL fusion in vivo. Various AF10 fusion-derivatives including NES-ENL were transduced to c-Kit-positive <t>hematopoietic</t> progenitors and transplanted into syngeneic mice. Primary NES-ENL leukemia cells were harvested from the bone marrow (BM) and transplanted into recipient mice. d Hierarchical clustering analysis of RNA-seq profiles of AF10 fusion-ICs. Normalized count data in various AF10 fusion-ICs was clustered using R ward D2 method. Source data are provided as a Source Data file.
Hematopoietic Stem Cell Culture Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Human+Hematopoietic+Stem+Cell+Media/Human+Hematopoietic+Stem+Cell+Media/pmc10082848-322-18-23
Average 91 stars, based on 1 article reviews
hematopoietic stem cell culture medium - by Bioz Stars, 2026-09
91/100 stars
  Buy from Supplier
human embryonic hematopoietic stem cell differentiation media with serum  (Celprogen Inc)
N/A
Human Embryonic Hematopoietic Stem Cell Differentiation Media with Serum. This product is also available without Serum Cat# M36092-19D This product would require pre-coated flasks with Human Embryonic Hematopoietic Stem Cell Extra-cellular Differentiation Matrix Cat# D36092-19
   Buy from Supplier
stemspan sfem ii human hematopoietic stem cell expansion media  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc stemspan sfem ii human hematopoietic stem cell expansion media
a Structures required for AF10 fusion-mediated myeloid transformation. Various AF10 fusion constructs were examined to assess their transforming ability of myeloid progenitors. HA-tag (indicated by red triangles) was fused to MTM and MTMT constructs. FLAG-tag (indicated by blue triangles) was fused to other AF10 fusion constructs. Dotted lines indicate protein-protein interaction. A schema of a myeloid progenitor transformation assay is shown at the top. Hoxa9 expression normalized to Gapdh in first-round colonies (left) is shown as the relative value of CALM-AF10 (arbitrarily set at 100%) (mean of two biological replicates). Colony-forming ability at the third- and fourth-round passages (right) is shown with error bars (mean ± SD of biological replicates, n ≥ 3). b Association of NES-AF10 fusion with ENL in the presence or absence of DOT1L. IP-western blotting (WB) analyses of the chromatin fraction of HEK293T cells [the parental clone or a DOT1L-knockout clone (dDOT1L)] transiently expressing the FLAG-tagged (indicated as f) NES-AF10´ fusion (fNES-AF10´) construct and Xpress-tagged (indicated as x) ENL (xENL) were performed. Co-purification of ENL was observed only in the presence of DOT1L. c Leukemogenesis by NES-ENL fusion in vivo. Various AF10 fusion-derivatives including NES-ENL were transduced to c-Kit-positive <t>hematopoietic</t> progenitors and transplanted into syngeneic mice. Primary NES-ENL leukemia cells were harvested from the bone marrow (BM) and transplanted into recipient mice. d Hierarchical clustering analysis of RNA-seq profiles of AF10 fusion-ICs. Normalized count data in various AF10 fusion-ICs was clustered using R ward D2 method. Source data are provided as a Source Data file.
Stemspan Sfem Ii Human Hematopoietic Stem Cell Expansion Media, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/Human+Hematopoietic+Stem+Cell+Media/stemspan+sfem+ii+human+hematopoietic+stem+cell+expansion+media/pm35108499-761-6-15
Average 90 stars, based on 1 article reviews
stemspan sfem ii human hematopoietic stem cell expansion media - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
human embryonic hematopoietic stem cell serum free un-differentiation media  (Celprogen Inc)
N/A
Human Embryonic Hematopoietic Stem Cell Serum Free Un-differentiation Media. This product is also available with Serum Cat# M36092-19US This product would require pre-coated flasks with Human Embryonic Hematopoietic Stem Cell Extra-cellular Un-differentiation Matrix Cat# U36092-19
   Buy from Supplier
human embryonic hematopoietic stem cell serum free media  (Celprogen Inc)
N/A
Human Embryonic Hemapoietic Stem Cell Serum Free Media. This Product is also available with Serum Cat# M36092-19S This product would require pre-coated flasks with Human Embryonic Hemapoietic Stem Cell Extra-cellular Matrix Cat# E36092-19 and Human
   Buy from Supplier
human embryonic hematopoietic stem cell serum free expansion media  (Celprogen Inc)
N/A
Human Embryonic Hematopoietic Stem Cell Serum Free Expansion Media. This product is also available with Serum Cat# M36092-19ES This product would require pre-coated flasks with Human Embryonic Hematopoietic Stem Cell Extra-cellular Expansion Matrix Cat# E36092-19
   Buy from Supplier
human embryonic hematopoietic stem cell un-differentiation media with serum  (Celprogen Inc)
N/A
Human Embryonic Hematopoietic Stem Cell Un-differentiation Media with Serum. This product is also available without Serum Cat# M36092-19U This product would require pre-coated flasks with Human Embryonic Hematopoietic Stem Cell Extra-cellular Un-differentiation Matrix Cat# U36092-19
   Buy from Supplier
human embryonic hematopoietic stem cell media with serum  (Celprogen Inc)
N/A
Human Embryonic Hematopoietic Stem Cell Complete Media with Serum. This product is also available without Serum Cat# M36092-19 This product would require pre-coated flasks with Human Embryonic Hematopoietic Stem Cell Extra-cellular Matrix Cat# E36092-19 and
   Buy from Supplier

Image Search Results


a Structures required for AF10 fusion-mediated myeloid transformation. Various AF10 fusion constructs were examined to assess their transforming ability of myeloid progenitors. HA-tag (indicated by red triangles) was fused to MTM and MTMT constructs. FLAG-tag (indicated by blue triangles) was fused to other AF10 fusion constructs. Dotted lines indicate protein-protein interaction. A schema of a myeloid progenitor transformation assay is shown at the top. Hoxa9 expression normalized to Gapdh in first-round colonies (left) is shown as the relative value of CALM-AF10 (arbitrarily set at 100%) (mean of two biological replicates). Colony-forming ability at the third- and fourth-round passages (right) is shown with error bars (mean ± SD of biological replicates, n ≥ 3). b Association of NES-AF10 fusion with ENL in the presence or absence of DOT1L. IP-western blotting (WB) analyses of the chromatin fraction of HEK293T cells [the parental clone or a DOT1L-knockout clone (dDOT1L)] transiently expressing the FLAG-tagged (indicated as f) NES-AF10´ fusion (fNES-AF10´) construct and Xpress-tagged (indicated as x) ENL (xENL) were performed. Co-purification of ENL was observed only in the presence of DOT1L. c Leukemogenesis by NES-ENL fusion in vivo. Various AF10 fusion-derivatives including NES-ENL were transduced to c-Kit-positive hematopoietic progenitors and transplanted into syngeneic mice. Primary NES-ENL leukemia cells were harvested from the bone marrow (BM) and transplanted into recipient mice. d Hierarchical clustering analysis of RNA-seq profiles of AF10 fusion-ICs. Normalized count data in various AF10 fusion-ICs was clustered using R ward D2 method. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: MOZ/ENL complex is a recruiting factor of leukemic AF10 fusion proteins

doi: 10.1038/s41467-023-37712-5

Figure Lengend Snippet: a Structures required for AF10 fusion-mediated myeloid transformation. Various AF10 fusion constructs were examined to assess their transforming ability of myeloid progenitors. HA-tag (indicated by red triangles) was fused to MTM and MTMT constructs. FLAG-tag (indicated by blue triangles) was fused to other AF10 fusion constructs. Dotted lines indicate protein-protein interaction. A schema of a myeloid progenitor transformation assay is shown at the top. Hoxa9 expression normalized to Gapdh in first-round colonies (left) is shown as the relative value of CALM-AF10 (arbitrarily set at 100%) (mean of two biological replicates). Colony-forming ability at the third- and fourth-round passages (right) is shown with error bars (mean ± SD of biological replicates, n ≥ 3). b Association of NES-AF10 fusion with ENL in the presence or absence of DOT1L. IP-western blotting (WB) analyses of the chromatin fraction of HEK293T cells [the parental clone or a DOT1L-knockout clone (dDOT1L)] transiently expressing the FLAG-tagged (indicated as f) NES-AF10´ fusion (fNES-AF10´) construct and Xpress-tagged (indicated as x) ENL (xENL) were performed. Co-purification of ENL was observed only in the presence of DOT1L. c Leukemogenesis by NES-ENL fusion in vivo. Various AF10 fusion-derivatives including NES-ENL were transduced to c-Kit-positive hematopoietic progenitors and transplanted into syngeneic mice. Primary NES-ENL leukemia cells were harvested from the bone marrow (BM) and transplanted into recipient mice. d Hierarchical clustering analysis of RNA-seq profiles of AF10 fusion-ICs. Normalized count data in various AF10 fusion-ICs was clustered using R ward D2 method. Source data are provided as a Source Data file.

Article Snippet: Human hematopoietic stem cell—CD34 + cells the from fetal liver (purchased from Cell Applications, INC.) were pre-cultured in Hematopoietic Stem Cell Culture Medium (Cell Applications, INC.) for 4 days and plated in methylcellulose-based medium (MethoCult TM H4435 Enriched, STEMCELL Technologies) in the presence of designated drugs for 6 days.

Techniques: Transformation Assay, Construct, FLAG-tag, Expressing, Western Blot, Knock-Out, Copurification, In Vivo, RNA Sequencing